How to Use the Protein Concentration Calculator
Calculate protein concentration using UV spectrophotometry:
Enter Absorbance A₂₈₀
Input UV absorbance reading at 280 nm.
Enter Extinction Coeff
Provide protein extinction coefficient (or default 1.0 for BSA/IgG).
Set Cuvette Pathlength
Specify cuvette pathlength (typically 1.0 cm).
View Concentration
Get instant protein concentration in mg/mL.
Key Features & Protein Spectrophotometry
Beer-Lambert Law Engine
Evaluates A = ε · c · l directly for purifications and assays.
Dilution Multiplier Support
Applies serial dilution multipliers for concentrated protein stock solutions.
100% Private Client Compute
Runs in browser memory with zero tracking or server logs.
Biochemistry Guide: Protein Quantification Methods
Protein concentration measurement is fundamental in biochemistry, cell biology, and biotechnology. Spectrophotometric measurement at 280 nm offers a non-destructive method for pure protein samples containing aromatic amino acids.
Frequently Asked Questions
?How is protein concentration calculated from A280?
Concentration (mg/mL) = A280 / (Extinction Coefficient × Pathlength) × Dilution Factor.