How to Use the DNA Copy Number Calculator
Calculate exact molecular copy count for qPCR standard curves:
Enter DNA Mass
Input nucleic acid sample mass in nanograms (ng).
Enter Sequence Length
Input plasmid or PCR product size in base pairs (bp).
Select Molecule Format
Choose dsDNA, ssDNA, or RNA format.
View Copies
Get absolute copy count and scientific notation for standard curves.
Key Features & Molecular Calculations
Avogadro Constant Precision
Applies Avogadro's constant (6.022 × 10²³) for molecular copy count.
qPCR Standard Curve Ready
Formats output directly in scientific notation for absolute qPCR quantification.
100% Private Client Compute
Runs in browser memory with zero tracking or server logs.
Genomics Guide: Absolute qPCR Copy Number Determination
In quantitative real-time PCR (qPCR), determining the exact copy number of viral genomes, bacterial genes, or transcript copies requires generating a 10-fold serial dilution standard curve of known DNA copy concentration.
Frequently Asked Questions
?What is the average molecular weight of 1 base pair of dsDNA?
The average molecular weight of a double-stranded DNA base pair is 650 g/mol (Daltons).