How to Calculate Dilution Factor (DF)
Perform lab dilution calculations step-by-step:
STEP 01
Enter Stock Volume V1
Input volume of concentrated stock solution added.
STEP 02
Enter Final Volume V2
Input total desired diluted solution volume.
STEP 03
Compute DF = V2 / V1
Calculate fold reduction ratio (e.g. 10× for 100 mL / 10 mL).
STEP 04
Find Solvent Volume
Solvent to add = V2 - V1 for exact bench preparation.
Key Features & Serial Dilutions
Ratio & Fold Expressions
Displays dilution factor both as fold multiplier (10×) and ratio (1 : 10).
Solvent Additions
Calculates exact diluent volume (V2 - V1) to mix into flask.
Serial Dilution Helper
Ideal for microbiology and ELISA assay step-down serial dilutions.
Laboratory Guide: Dilution Factor (DF)
The Dilution Factor (DF) defines how many times a solution is diluted:DF = V2 / V1 = C1 / C2
?
Frequently Asked Questions
?What does a 1:10 dilution mean?
A 1:10 dilution means 1 part stock solution combined with 9 parts solvent for a total of 10 parts final volume (DF = 10).