How to Use the Cell Dilution Calculator
Calculate cell culture passaging and seeding dilutions:
Enter Stock Conc C₁
Input current stock cell density from hemocytometer counting.
Enter Target Conc C₂
Specify desired cell seeding density for culture plate or flask.
Enter Target Volume V₂
Provide final working volume required in mL.
View Pipetting Volumes
Get exact stock cell volume (V1) and culture media volume required.
Key Features & Cell Passaging Math
C1V1 = C2V2 Conservation
Applies mass balance conservation equations for cell suspensions.
Culture Media Volume Calculation
Displays exact volume of fresh growth media to mix with cell stock.
100% Private Client Compute
Runs in browser memory with zero tracking or server logs.
Cell Culture Guide: Passaging & Seeding Calculations
In cell biology laboratories, accurate cell seeding density is critical for reproducible cell culture experiments. Over-seeding leads to rapid contact inhibition and nutrient exhaustion, while under-seeding can cause lag phase prolongation or cell death.
Frequently Asked Questions
?How do I calculate cell dilution?
Multiply target concentration C2 by target volume V2, and divide by stock concentration C1 to find stock volume V1.